Anatomical distribution of biotin-binding protein/avidin in the hen oviduct.
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چکیده
منابع مشابه
Glucocorticoid binding in the hen oviduct.
[(3)H]Triamcinolone acetonide (15nm) was incubated with cytosol (150000g fraction) prepared from oviducts of egg-laying hens. The extent of steroid binding, as determined by charcoal assays, was greatest between 2-4h at 4 degrees C. A similar time curve was obtained when cytosol preparations were first fractionated with (NH(4))(2)SO(4) before labelling. The addition of 10mm-Na(2)MoO(4) or 10mm-...
متن کاملDistribution of estrogen receptors in subfractions of hen oviduct chromatin.
Hen oviduct chromatin was digested with DNase II and separated into two fractions. The MgCl2 insoluble chromatin fraction (43% of the total DNA) was enriched in nucleosome-like particles, which sedimented at 11 S and contained 185 base pairs of DNA. The MgCl2 soluble chromatin fraction (5% of the total DNA) was characterized by 5 S and 14 S peaks in sucrose gradients; Estrogen receptors in the ...
متن کاملImmunoreactive avidin in the hen oviduct mucosa.
diameter)in the gland and small(200 to 700 nm) in the epithelium. Epithelial cells containing secretory granules had a strong resemblance to those of the protodifferentiated gland cells appearing in the magnum of chicks pretreated with hormones. No avidin was observed in any epithelial goblet cells or ciliated cells. The findings paralleled those obtained by biotinylatedenzymes affinity cytoche...
متن کاملThe polyA regions of hen oviduct RNA.
Total RNA from hen oviduct has been hydrolysed with a mixture of T(1) and pancreatic ribonucleases. Poly(A) tracts in the digestion product have been isolated by binding to oligo(dT) cellulose. Of the four major ribonucleotides, the product has been shown to contain only adenylic acid. When separated on polyacrylamide gels, the poly(A) gave two peaks corresponding to average apparent lengths of...
متن کاملEnzymatic Sulfation of Mucopolysaccharides in Hen Oviduct
An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1988
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.21.463